Your English writing platform
Free sign upSuggestions(2)
Exact(3)
Similar to MRP4, the identification of MRP5 was mainly based on expression sequence tag data analysis followed by cDNA fragment cloning [55], [90].
Previous investigations based on expression sequence tags (ESTs) have documented that the tetraploid (2 n = 48) species P. ginseng and P. quinquefolius have experienced two rounds of WGD [ 34, 36].
However, with the exception of Oct4, these factors are not highly expressed maternally in oocytes, and only increased by zygotic transcription during preimplantation, based on expression sequence tag (EST) frequencies in Unigene cDNA libraries and microarray data from oogenesis to preimplantation development (5).
Similar(57)
Since Affymetrix 3' expression arrays were designed based on expressed sequence tags, many probesets map to intergenic regions downstream of genes.
While studies to characterize the overall gene expression of parasitic nematodes based on expressed sequence tags (ESTs) have become relatively common in recent years [e.g., [ 23- 32]], very few reports dealt specifically with ESTs of genes that code for secretory proteins [ 13, 33, 34], and none combined expression analyses of all genes with detailed sequence mining approaches.
The avian β-keratins were originally grouped into four subfamilies (claw, feather, feather-like, and scale β-keratins) based on expression profiles and sequence heterogeneity [ 29- 31].
Since the identification of the genes putatively mapped on D genome (or on CS_5AL-10) was based on expression data, the sequences not confirmed by mapping experiment probably represent genes whose expression is controlled by factor(s) carried on D genome (or on CS_5AL-10).
Based on expression data and corroborative sequence data, wild derived strains (MOLF/EiJ, CAST/EiJ, PWK/PhJ, and PWD/PhJ) and Swiss mice (FVB/NJ and SJL/J) lack the insertion.
Finally, we demonstrate that sequence harmonization based on expression host tRNA pools, designed to mimic ribosome movement of the original organism, can significantly increase the folding of the encoded polypeptide.
All shRNA and sgRNA sequences are shown in Supplementary Data 8. Transduced HSPCs were sorted based on expression of GFP or Cerulean.
Specific primers for rapid amplification cDNA ends (RACE) were designed based on the expression sequence tag (EST, BM173953) to amplify the 3′- and 5′-ends of hc-serpin.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com