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Analysis of gene function during root endosymbioses requires reverse genetics approaches based on expression perturbation experiments.
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In Xenopus, XVLG1, a VASA homolog, appears to be involved in general cell specification and proliferation, rather than only in germline development, based on the expression pattern and perturbation of protein function (Ikenishi and Tanaka, 1997; Ikenishi and Tanaka, 2000).
We have used gene expression data to build two models: a knowledge-driven model based on gene expression changes following gene perturbation experiments, and a data-driven mathematical model derived from time-course gene expression data recovered from wild-type animals.
Perturbation based on a deterministic perturbation vector set can avoid extensive signaling and feedback overhead [9].
The second approach (sometimes used in combination with ChIP-seq) is based on genetic perturbation of identified TFs and large scale analysis of expression level changes.
These models can be used in combination with an algorithm that can predict the activity of a backbone node based on gene expression changes attributable to a perturbation of the biological process.
In [27] an approach based on XML perturbation is presented.
The analysis is based on singular perturbation techniques.
Theoretical curve based on the perturbation calculation is shown by the solid curve.
In all variants of AFM, the objective function is based on weighted perturbation data.
The solution to the governing differential equation is proposed based on nonlinear perturbation expansions.
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