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Phylotranscriptomics, or the inclusion of transcriptomic data into phylogenetic studies, differs from other methods in that it is based on expressed mRNA sequences and does not require previous knowledge of specific gene regions (Cronn et al. 2012; McCormack et al. 2013).
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However, tumor specific signatures of alternative hyper-splicing are still lacking, primarily since alternative splicing studies are based on expressed sequence tags (EST) or mRNA sequences [8], [9].
The collected target mRNAs were further screened based on abnormally expressed mRNA profiles.
As shown in Figure 1a, hierarchical clustering based on differentially expressed mRNAs revealed clear separation of the two groups where the cancer tissues clustered separately from the normal tissues, except for one branch of the cancer group representing one cancer sample, which was misclassified to the normal group.
We further mapped IPA based interactions between differentially expressed mRNAs and miRNAs in HGSC.
Second, the significant anti-correlation between miRNA-mRNA pairs was determined based on the expression level of each aberrantly expressed miRNA and each aberrantly expressed mRNA.
We employed IDEG6 (http://telethon.bio.unipd.it/bioinfo/IDEG6/) to identify differentially expressed mRNAs based on their relative abundance which was reflected by total count of individual sequence read between the two libraries.
A CNC network was built for the ten differentially expressed lncRNAs and hundreds of differentially expressed mRNAs based on the degree of correlation (see Additional file 5).
Annotation of alternative splicing has traditionally been based on cDNA (expressed sequence tags (EST), mRNA) sequence data from public repositories such as dbEST, RefSeq, and the Mammalian Gene Collection.
Hia was not identified as an IVI gene in our initial study [20], and consistent with this finding, was subsequently was found to be constitutively expressed based on our mRNA analysis (data not shown).
Quantitative analysis of cytokine messenger RNA (mRNA) transcription based on the comparison of the cytokine with the housekeeping gene feline glyceraldehyde-3-phosphate dehydrogenase (GAPDH), providing universally expressed mRNA.
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