Diagnosis is based on exclusion.
Their brotherhood is based on exclusion.
In this article, we extracted peptidome fractions (molecular weight <5,000 Da) from 92 individuals using the well-established and reproducible one-step peptidome enrichment method based on size exclusion chromatography (SEC) [22], [23] and provided them to the label-free mass spectrometric quantification analysis combined with the statistical analyses on Expressionist proteome server platform.
A second purification step, based on size exclusion chromatography, was then utilized, being effective in the elimination of other previously identified contaminating proteins.
Based on size exclusion chromatography, major antioxidative peptides in NS-8GE and SS-8GE had molecular weight of 1,170 and 750 Da, respectively.
Milligram amounts of construct 4 NA per litre of insect cell culture were obtained from large scale expressions without obvious destabilization of the NA tetramer (based on size exclusion chromatography) or breakdown products (based on SDS-PAGE and western blot) as has previously been the case[12], [15], [16].
Recently, Li et al. prepared mutants of the dimeric cockroach allergen Bla g 2. One constructed mutant appeared to be monomeric, based on size exclusion chromatography analysis, and it induced less β-hexosaminidase release from mast cells than the authentic Bla g 2 [9].
It has typically been reported to have an apparent molecular mass between 250 and 350 kDa, based on size exclusion chromatography (SEC) and analytical ultracentrifugation (AUC) experiments (see, for example, refs (20− 20−).
The determination of the oligomeric state of proteins is normally based on size-exclusion chromatography, ultracentrifugation, X-ray scattering or dynamic light scattering.
The hydrogenase was obtained as a homogeneous protein of MW ∼150,000 Da (based on size-exclusion chromatography, data not shown), also similar to that of the native enzyme, and contained the four different protein subunits (Fig. 6a) [11].
