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Immunizations were performed by GenScript (USA) based on established protocols [49].
Based on established protocols [18], types I, III and V collagens were isolated from the skin of chicken wings purchased at the supermarket and Xenopus laevis purchased from Xenopus Express, Inc. (Brooksville, FL).
Enumeration of SISH signals was based on established protocols [ 17].
The IVF and ICSI procedures were based on established protocols [ 26].
2) We have provided additional references in the Materials and methods section, reinforcing that our sample preparation was based on established protocols.
To determine the genome size of S. melliferum IPMB4A, we performed pulsed-field gel electrophoresis (PFGE) based on established protocols [ 37, 38].
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Microarray hybridisation and data acquisition were carried out by ARK-Genomics (Roslin Institute, University of Edinburgh) using 23 K Affymetrix GeneChip Porcine Genome Arrays based on established ARK-Genomics protocols (http://www.ark-genomics.org/protocols).org/protocols
Based on established adipogenic differentiation protocol with specific medium [23], Icaritin at 0 M (indcution group), 10−16 M (low dose group), 10−14 M (middle dose group), 10−12 M (high dose group) and parent compounds in EF (EF1 ∼ EF7) at 10−14 M was added to the medium over the full course of differentiation.
Our protocol is based on established methods [22], [3], [4], [7] but substantial modifications to the available rat protocols had to be introduced (Table 2).
The development of cryopreservation protocols for cell-based therapeutics have been based on established strategies to balance the "two-factors" of cryoinjury that influence cell recovery.
The study protocol was based on established guidelines [ 21- 23] and consisted of detailed practical instructions on topics like recruitment, location and settings, tape equipment and duration of sessions.
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based on established tools
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based on established prejudices
based on established criteria
based on established guidelines
based on established cut-offs
based on modified protocols
based on established rules
based on established values
based on established principles
based on established facts
based on established metrics
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based on established algorithms
based on established theories
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