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We disregarded sites at which more than 10% of individuals had fewer than 50 reads and therefore could not be down-sampled, so estimates of heteroplasmy from all remaining sites were based on equal coverage on an adequate sample size.
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The RAD libraries gave about a 15-fold better coverage depth in the sampled genomic region than the semirandom (SR) library, based on equal numbers of uniquely aligning reads accepted after quality filtering.
Will it infringe our commitment to institutions based on equal dignity?
It is a totally phony argument based on equal parts of surmise and bigotry.
‡The t-test was calculated based on equal variances.
The analysis was based on equal amount of cells and equal amount of RNA per cell.
Due to the difficulty in assembly of the non-coding region, the total lengths of the maxicircles were estimated based on coverage values of the coding region, assuming relatively equal coverage throughout the entire maxicircle and a population of maxicircles of equal size.
We scored these epitopes based on the allele coverage and total coverage score, which was in general normally distributed.
Researchers we interviewed adjust the coverage threshold based on the overall coverage and their intuition without any visual aids.
But that calculation is based on individual coverage for the worker alone, not family coverage, which is much more expensive.
But I have no doubt that it had some impact, just based on the coverage".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com