Sentence examples for based on enzyme sequences from inspiring English sources

Based on enzyme sequences, precursors for synthesis of the branched-chain fatty acids could be generated as intermediates in breakdown of the amino acids leucine, isoleucine and valine.

The formation of synthetic polymers with a better control of the sequence of units along a chain can happen through polymerizations based on enzyme-like catalytic activity.

Relying on a metabolic network rather than a PPI network is highly advantageous as metabolic networks are readily available for hundreds of species based on cross-species enzyme sequence homology (Kanehisa and Goto, 2000), while large-scale PPI networks depend on costly high-throughput experiments that are currently available for only a few organisms.

Here, we sought to investigate the methylation status of the CpG islands of MYCT1 and mRNA levels by bisulfite-specific PCR (BSP) based on sequencing restriction enzyme digestion, reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR).

Ligation-mediated methods are based on digesting sequences with restriction enzymes, ligating sequences into a plasmid, transforming bacteria with plasmids and screening for strains containing the flanking regions.

We first decided to check for nearest relatives/neighbors in databases using phylogenetic analyses of the genes for the putative chitinolytic enzymes (based on predicted protein sequences).

The discovery of enzymes capable of deaminating 5-methylcytosine and 5-fluorocytosine reveals the difficulty of defining the substrate/sequence boundaries of enzymes based on simple sequence similarity to proteins of known function.

The design of the probe and the choice of the appropriate restriction enzyme were based on the sequence of the specific vectors.

A soil metagenomic library was constructed and two screening methods based on protein sequence similarity and enzyme activity were investigated to isolate novel XI encoding genes.

These enzymes have been classified into eight different classes (A H) based on their sequences and biochemical features.

The first PCR based characterisation method based on restriction enzyme analysis of insertion sequence 1311 ISS 1311 PCR-REA) and Heat shock protein 65 gene, used limited amounts of DNA but could not distinguish between most isolates [ 8, 9].