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Conclusions could be drawn for the process of lignin degradation based on enzyme expression values as assessed by proteomics and the amount of chemical species related to these enzymes, assessed by metabolomics.
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E. coli was found to be an efficient biosynthetic vessel for producing prolyl 4-hydroxylated and α-GlcNAcylated Skp1 based on enzyme co-expression.
In addition to visualizations, a well-developed interface could integrate suggestions for genes based on enzyme activity and evidence of heterologous gene expression so that the user can seamlessly transition from pathway discovery to the initial build phase.
A step to more realistic benchmark data was made in 2003 [ 28], generating simulated gene expression data using equations based on enzyme kinetics (for use of these data in method evaluations, see e.g. [ 8, 29]).
Other common types of sensors are based on enzyme reactions.
More importantly for enzyme discovery, EC assignments can facilitate word searches based on enzyme function.
FunnyBase links annotated genes to enzymes in KEGG pathways based on enzyme commission (EC) numbers.
Medium had no effect on enzyme expression.
Scaling factors of different enzymes based on CYP expression systems were calculated and shown in Table 3.
Finally, our results only draw conclusions based on the expression of the enzymes; for the aforementioned reasons, we were unable to determine activity and/or substrate availability.
Based on the expression of endogenous enzymes in the HS/heparin pathways of CHO-S cells, human N-deacetylase/N-sulfotransferase (NDST2) and mouse heparan sulfate 3-O-sulfotransferase 3-O-sulfotransferase 3-O-sulfotransferase 1lly into CHs3st1t cells genesng in suspension culture.
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