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Taken together, the described results show that selection protocols based on enzymatic activity can be designed for this enzyme class which should be of importance for future protein engineering attempts.
This is because: (1) Different carboxylesterases show substrate or pI value overlap; (2) Various labs isolated the same carboxylesterase independently and assigned it a different name based on enzymatic activity; (3) There is a significantly larger number of carboxylesterase genes in rodents compared to humans, and this makes mouse/human ortholog assignment challenging.
The system is tested in several scenarios based on enzymatic activity.
Of course, detection based on enzymatic activity does not mean that the amount of protein produced would be biologically meaningful in all cases.
Alternatively, computational programs [e.g., Meteor Nexus (Lhasa Limited)] or in vitro metabolism assays may be used to predict metabolites based on enzymatic activity.
In the case of the fungus P. chlamydosporia, it has been proposed that the ovicidal effect is based on enzymatic activity, but with respect to B. Thuringiensis, the mechanism of action is unknown.
Similar(53)
Its major biochemical characteristics based on enzymatic activities in the in vitro system were summarized in Table 1.
Current approaches for evaluating acyltransferase activity are mainly based on enzymatic assays monitored by quantification of radioactive compounds via thin-layer chromatography [15] [18].
No differences were detected demonstrating that the decrease in the enzymatic activity of the Ile170Val TPI variant is based on reduced enzymatic activity per se (data not shown).
The analytic methods were based on enzymatic colorimetric assays [ 19].
FFA and TG assays are based on enzymatic reactions.
More suggestions(16)
based on enzymatic maceration
based on enzymatic chemiluminescence
based on enzymatic mechanism
based on suspicious activity
based on enzymatic silver
based on historic activity
based on enzymatic digestion
based on enzymatic inhibition
based on enzymatic transesterification
based on enzymatic detection
based on enzymatic nanolithography
based on spiking activity
based on enzymatic generation
based on enzymatic synthesis
based on enzymatic resolution
based on sexual activity
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