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Its major biochemical characteristics based on enzymatic activities in the in vitro system were summarized in Table 1.
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Taken together, the described results show that selection protocols based on enzymatic activity can be designed for this enzyme class which should be of importance for future protein engineering attempts.
This is because: (1) Different carboxylesterases show substrate or pI value overlap; (2) Various labs isolated the same carboxylesterase independently and assigned it a different name based on enzymatic activity; (3) There is a significantly larger number of carboxylesterase genes in rodents compared to humans, and this makes mouse/human ortholog assignment challenging.
The system is tested in several scenarios based on enzymatic activity.
Of course, detection based on enzymatic activity does not mean that the amount of protein produced would be biologically meaningful in all cases.
Alternatively, computational programs [e.g., Meteor Nexus (Lhasa Limited)] or in vitro metabolism assays may be used to predict metabolites based on enzymatic activity.
We also indicate whether the alteration is known to be clinically pathological based on an observation of a known symptomatic individual or predicted to be pathological based on enzymatic activity or putative disruption of the protein structure.
In the case of the fungus P. chlamydosporia, it has been proposed that the ovicidal effect is based on enzymatic activity, but with respect to B. Thuringiensis, the mechanism of action is unknown.
Current approaches for evaluating acyltransferase activity are mainly based on enzymatic assays monitored by quantification of radioactive compounds via thin-layer chromatography [15] [18].
The analytic methods were based on enzymatic colorimetric assays [ 19].
FFA and TG assays are based on enzymatic reactions.
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