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These results have implications for the design of sensors based on encapsulated binding sites.
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Anti-NadA mAbs were selected based on their binding site on NadA constructs using dot blot (Supplementary Fig. S5).
In addition, the electrostatic potential binding energy and similarity between the binding sites can be calculated based on the binding pose of the ligand.
However, previous studies identified the protein Scyl1 binding partner 1 (Scyl1basedbased on its binding to Scyl1 [30].
As shown, the results based on continuous binding scores outperform those based on discretized data.
ABFs/AREBs were isolated based on their binding to ABRE.
Here we compare the effects of two direct immobilization methods on ligand binding, kinetics, and signal transduction of reagentless fluorescent biosensors based on engineered periplasmic binding proteins.
The theoretical model is based on a tight binding Hamiltonian with Hubbard term.
Based on the calculated binding affinity to MHC, epitopes were selected for ELISpot assays (IC50 < 50 nM, Supplementary Table 1).
This network is primarily based on actin-binding activities.
Predictions of binding affinity to a given substrate are based on similarities in binding-site signature.
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