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We note that this classification is based on embryonic lethality after par-1 RNAi treatment alone, without a control for background levels of embryonic lethality.
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Based on the embryonic lethality of hep mutants it is obvious that Mkk4, which is expressed during embryonic development, cannot substitute for Hep function in this process.
Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality.
For the exposure worms were reared during pre-exposure phases at 15°C, a temperature with no effect on embryonic lethality.
The data were analyzed using a generalized linear model with a quasi-binomial error structure to test the effect of strain on embryonic lethality.
This could be of particular interest in translational research, particularly the one based on synthetic lethality.
These Brca1 mutant mice thus appeared to have a more complete rescue of embryonic lethality based on genetic background differences alone than Xu et al. were able to obtain by introducing Trp53 haploinsufficiency.
A new version of targeted therapy is based on a so-called "synthetic lethality" principle 61.
In each case, we found embryonic lethality as expected.
(Based on the success of the program, lethality assessments became Oklahoma state law in 2014).
Additional alleles were recovered based on their failure to complement the recessive lethality.
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