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MSSCP is based on electrophoretic separation of single-stranded DNA in native conditions, at sequentially changed gel temperature.
The classical method is based on electrophoretic separation of cleaved substrates on SDS-PAGE, using Atg8 substrates fused with a tag C-terminal to the cleavage site.,, The fused tag allows the detection of cleavage based on either the change of the size of the substrate or the appearance of the C-terminal cleavage products.
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To this end, we performed peptide competition assays based on electrophoretic mobility shift assay.
Figure S2, zeta potential of PHMB-stabilized silver nanoparticles based on electrophoretic mobility of PHMB-stabilized silver nanoparticles.
bDetermination based on electrophoretic mobility.
The ζ-potential of the particles was measured with a Zetaseizer Nano-ZS (Malvern Instruments) based on electrophoretic light scattering.
The aim of the present paper is to provide an up-to-date overview and critical discussion of the integration of these CNTs-based strategies in analytical processes based on the electrophoretic separations employed so far, both conventional CE and microchip electrophoresis (ME).
The influences of some crucial parameters including buffer concentration, pH value, micelle concentration and applied voltage on electrophoretic separation were investigated.
We observed that oocyte DNA fragments exhibit a clearly quantized size distribution with a periodicity of 10 to 11 bp on electrophoretic separation.
The SMSreg has been used to identify patients lacking the MS-typical oligoclonal band (OCB) pattern seen on electrophoretic separation of immunoglobulin gamma (IgG).
In contrast, DNA released by necrosis is nonspecifically digested and thus exhibits smears on electrophoretic separation with fragment sizes about 10,000 bp [ 10].
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