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The transmembrane region has not been resolved experimentally and is represented by geometric shapes of approximate size and shape based on molecular mass and positioned in close proximity to one another, based on electron tomography.
In this study, we have used an integrated approach based on electron tomography, single-particle electron microscopy, and crosslinking mass spectrometry to determine the structure of a major scaffold motif of the human NPC, the Nup107 subcomplex, in both isolation and integrated into the NPC.
Unsealed lipid edges and incomplete β-barrels are a surprising by-product of this membrane perforation by incomplete CDC oligomers, as was recently suggested based on electron tomography of various pneumolysin assemblies (Sonnen et al., 2014).
Current thinking based on electron tomography of immature virions (Wright et al., 2007; Carlson et al., 2008; Briggs et al., 2009) and bud sites (Carlson et al., 2008) is that ESCRTs and especially ESCRT-III play important roles both in completing the viral sphere (that is only 2/3 covered by polymerized Gag) and in severing its connection to the cell.
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We present experiments based on electron channelling contrast imaging (ECCI) as refined application of the scanning electron microscope (SEM) and new focused ion beam (FIB) technique like FIB crack initiation and FIB tomography which give detailed information about crack initiation and the interaction of short fatigue cracks with precipitates and grain boundaries as microstructural barriers.
CAC scores were derived based on electron-beam computed tomography.
Based on cryo-electron tomography, Cheng et al. reported six raw clathrin fullerene cages.
At the Second Annual Conference on Electron Tomography, held in Amsterdam in 2001, we proposed that electron tomography data should be shared in a manner analogous to structural data at the protein and sequence scales.
A multiscale analysis based on microcomputed tomography, histology, polarized light microscopy, backscattered electron microscopy, small angle X-ray scattering and nanoindentation was used to characterize bone volume, collagen fiber orientation, mineral particle thickness and orientation, and local mechanical properties.
In this paper, model-based control is discussed when the observations are based on diffuse tomography such as impedance tomography.
A simulation, based on computed tomography scans, is presented.
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