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Because several indels differentiate repeats within and between species, I constructed a neighbor joining tree based on distance using a model that considers adjacent indels as a 5th nucleotide state.
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Bootstraping was performed by sampling with replacement of each of the 256 tetranucleotide frequencies using Swaap PH 1.0.1 [ 39], and phylograms created based on distance matrices using Phylip 3.5 [ 40], reviewed via Treeview [ 41], and displayed using midpoint rooting with Paup 4.0b10 [ 42].
Phylogenetic trees were constructed based on distance data using the neighbor-joining method (Saitou and Nei 1987) implemented in ClustalX.
Fig. 2 Cluster dendrograms based on distance matrices using Bray-Curtis dissimilarity of standardized genera abundance data based on 16S rDNA amplicons from extracted DNA (a) and RNA (cDNA) (b) of unfertilized soil-compost mixture (UC) at days 0, 35, 48 and 160 by the UPGMA agglomeration algorithm (for details see M&M section).
Phylogenies were constructed based on distance matrices using Paup 4.0b10 [ 42].
Neighbor-joining trees were generated based on distance matrices using the Jones-Taylor-Thornton model.
When one of the paired-end reads mapped to the reference and the other did not, MAQ tries to align the read in the expected region (based on the distance) using the SW algorithm, allowing us to identify significant indels in our sample, in particular specifically recurrent indels that originate from population diversity and not sequencing error.
Biological processes in the GO annotations were used and profiles were clustered based on Euclidean distance using Ward's method.
(F ) Using Jalview, a distance tree relating the various IF2 homologs was calculated based on average distance using the percent identity.
Cluster analyses were performed with the significantly differentially expressed miRNAs using the hierarchical cluster analysis program within TMeV, based on Euclidean distance using average linkage clustering methods.
MANOVA with 999 permutations was used to test significant difference between sample categories based on Bray Curtis distance using Adonis (Anderson, 2001).
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