Sentence examples for based on direct sequence from inspiring English sources

In addition to tree-based assignment we used criteria based on direct sequence comparison.

The systems were developed based on direct sequence, indirect sequence, Petlyuk and side stripper designs.

Operating at 2.4 GHz, it was based on direct sequence spread spectrum modulation (DSSS) and supported a maximum data rate of 2 Mbps.

Since the best match was based on direct sequence comparison with other conspecific ecotypes, the analysis only counted species with multiple ecotypes per species.

The increasingly high-throughput nature and declining costs of second-generation DNA sequencing have provided a durable solution for transcriptome analysis based on direct sequence evaluation through transcript discovery, identification of the transcriptional structure of a gene, detection of alternate splicing patterns, and quantification of expression levels [ 7, 8].

We assessed the ease of amplification and sequencing for these markers, examined the extent of the barcoding gap between intra- and inter-specific variation by pairwise distances, evaluated successful identifications based on direct sequence comparison of the "best close match" and the construction of a phylogenetic tree.

Physical gaps were closed based on direct sequencing of selected clones.

In order to further validate the results from the approach based on direct sequencing of PCR products from genomic DNA, PCR products from several loci were cloned.

These are usually based on direct sequencing, often preceded by denaturating high-performance liquid chromatography (dHPLC), single strand conformational polymorphism (SSCP) or Tetra-ARMS-PCR assay [ 8- 10].

Subsequently, once a Neandertal genome sequencing effort based on direct sequencing of DNA extracts was initiated, the construction of tagged libraries for high-throughput sequencing was established in the clean room environment in our laboratory (Briggs et al, 2007).

Real-time polymerase chain reaction (qPCR) and microarrays are the two well-established miRNA-profiling approaches for miRNA detection; next-generation sequencing (NGS) is another new method based on direct sequencing.