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The method is based on direct fluorescence detection of fluorophore-labelled protein before and after conjugation.
Briefly, following incubation with the appropriate antibody, membranes were scanned using the LI-COR Odyssey (LI-COR, Lincoln, NE, USA) and quantified using Odyssey 2.0 software based on direct fluorescence measurement.
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These observations were based on: direct visualization of fixed or live cells using confocal microscopy; and by quantifying the cellular fluorescence in live cells using flow cytometry.
A rapid procedure based on direct extraction and HILIC separation of aliskiren (ALI) degradation product – 3-amino-2,2-dimethylpropanamide (ADPA) with fluorescence detection has been developed.
'Tree' is an ordinary, descriptive concept based on direct observation.
The sensitivity analysis was based on direct treatment costs.
INLA is based on direct numerical integration instead of simulations.
Scoring is based on direct observation of performance.
We conduct our analysis based on direct clinical comparisons.
Searching based on direct citations is not sufficiently comprehensive.
Information for patients should therefore be based on direct standardization.
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