The raw sequencing reads have been mapped to the human reference genome using a consensus strategy based on different sequencing platforms, and the output is in binary BAM format [ 34].
These results indicated that SNP concatemers based on different reference sequences behave differently in phylogenetic analysis, emphasizing the critical importance of selecting the proper reference sequence, and CS is superior to H37Rv when it is used as the reference sequence in phylogenetic analysis of MTBC.
AtFYVE domains can be divided into two distinct groups based on different consensus sequences identified via CLUSTALW multiple sequence alignment (Fig. 5).
These are carried out by investigating various effects based on different stacking sequences, stiffness and span-to-thickness ratios, mode shapes and boundary conditions.
Based on different reference sequences, three sets of polymorphic marker between 'Chinese Antique' and 'AL1' were identified respectively by pairwise grouping from each genotype.
Table 1 gives five alternative names that are legal for a SNP, where the coordinate systems are based on different reference sequences.
In addition, phylogenetic trees based on different protein sequences display different tree topologies, as shown in the Figure (panel B), which indicates the existence of multiple evolutionary pathways for different regions of the genome.
The deeper branching and distinctness of Bradyrhizobiaceae and Methylobacteriaceae from other Rhizobiales is also strongly supported by phylogenetic analyses based on different gene sequences and conserved indels in many proteins [ 1, 17, 45].
These proteins indicate that the Rickettsiales constitute a distinct clade within the α-subdivision, which is in accordance with phylogenetic analyses based on different gene sequences [ 2, 17, 43, 50].
The silencing efficiency of shRNA varies based on different shRNA sequences, shRNA expression cassettes, and cancer cell lines [ 40, 41], which is a disadvantage because it requires comprehensive bioinformatic and experimental data to select the most effective shRNA against a particular target.
