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Some size separation methods based on density gradient ultracentrifuge [40] and pH selective precipitation [39] were put forward, but further purification process is needed for these methods.
The enrichment can be based on density gradient centrifugation, flow cytometry, immunomagnetic beads, and membrane filtration [ 11].
Subsequent methods incorporated the simultaneous isolation of different hepatic cell populations based on density gradient centrifugation with Stractan [ 9].
To date, plasma membrane isolation from Drosophila tissues has been based on density gradient centrifugation [ 36- 38], and to the best of our knowledge the use of 2PAP has not yet been reported for this model.
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Although the subcellular organelle purification method based on density gradients makes it difficult to avoid contamination by unwanted components, it is still a common and efficient method to enrich our interested organelles.
Engineering-oriented simulations of quantum mechanical tunneling are often based on density-gradient (DG) theory.
To achieve this goal, we isolated PLSs through a fractionation procedure based on density-gradient centrifugation.
First-principles calculation based on density functional theory with the generalized gradient approximation (GGA) were carried out to investigate the energy band gap structure of this special structure.
The generalized gradient approximation based on density functional theory is used to study the effects of the benzyne group on the M3N@C80 (M = Sc, Y) clusters.
First-principles calculations based on density functional theory within the generalized gradient approximation (GGA) have been performed for the rutile M/SnO2 (M=Ge, Mn) (110) surfaces.
All calculations were performed using STATE program package [8] based on density functional theory within a generalized gradient approximation, and we employed an exchange-correlation energy functional proposed by Perdew et al. [9].
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