The activity of silencing was estimated based on deletion effects of the Sir complex components (Sir2, Sir3, and Sir4) and Set1 (see Methods).
The sites are not involved in fmgA regulation based on 5' deletion analysis of the promoter region fused to a lacZ reporter [ 54].
A number of studies based on deletion and site-directed mutagenesis demonstrated that the central helices in these BCL-2 family proteins are required for pore formation and for both cytoprotection and apoptosis induction [29], [30], [31], [32], [33].
The final category of algorithms is based on deletion inference from genotype data with a familial structure.
In an attempt to solve this problem we developed a novel virus strain, ASFV-G-Δ9GL/ΔMGF, based on the deletion of all genes deleted in ASFV-G-Δ9GL and ASFV-G-ΔMGF.
The first model was based on conditional deletion of the p53, resulting in induction of mammary tumours after 45 weeks.
We have previously developed the first prototype of an oncolytic influenza A virus, based on a deletion of the viral non-structural NS1 gene.
Biological networks are robust [1], and we predicted that the differences between the healthy and disease state for complex and polygenic disease would not be based on the deletion of nodes (genes or proteins) or even links (gene or protein interactions), but rather in more subtle changes in the weight of the interactions.
First, we confirm several previous findings (20 – 23) based on the deletion of these kinases in multiple tissues, showing that when restricted to the β cell, loss of LKB1 exerts markedly different effects on insulin secretion and glucose metabolism compared to the loss of AMPK (Figs. 1– 3).
Recent paper by Taylor et al. (Taylor et al., 2014) describes a new method called Digital Deletion Detection, based on enrichment of deletions by wild-type specific restriction digestion, massive single-molecule PCR in microdroplets, followed by next generation sequencing of the PCR products.
