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An alternative approach to identify prognostic signatures is based on defining gene sets involved in a biological process or specific environmental condition that is suspected of influencing treatment response or patient outcome.
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Nevertheless, the present screening protocol could be used in the future to define different MSC populations based on a defined gene panel.
Molecular variation based on functionally defined genes underlying specific biochemical or physiological functions will provide the next generation of molecular markers for pearl millet.
Another strategy is based on defining subtypes for a complex trait using gene expression profiles.
In addition, defining gene families based on features of demonstrable functionality (domains) should provide a more functionally cohesive grouping compared to assigning genes to families based on often rather arbitrary sequence similarity cut-offs.
Additionally, GSEA for the on-target signatures was conducted using Fisher's exact test based on gene sets defined by KEGG pathways as annotated by the Bioconductor package KEGG.db version 2.2.0.
We applied the UBC molecular classifier based on gene expression defined by Sjodahl et al [ 12] to identify lines most representative of the taxonomical groups proposed.
Briefly, we defined nuclear genes based on the gene model coordinates defined in SpBase Build 6 based on the Spur v3.1 genome assembly (SpBase.org).org
For each pathway, we considered the subset of genes overlapping with CNVs based on the gene areas (defined above) and represented it with the corresponding set of CNV-gene pairs.
S and Pr could be defined for stimuli based on gene set or protein phosphorylation activation.
Usually, class discovery in cancer studies is for the purpose of determining whether discrete subsets of a disease entity can be defined based on the gene expression profiles.
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