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The list of EBPs that was obtained in a BLAST search using the Sigma-54 interaction/activator domain (PFAM: PF00158), was initially filtered using two criteria based on cut off value (see methods).
Due to a high level of polymorphism between the RXLR16 protein sequences in Waco9 and Emoy2, the blast hit obtained was excluded based on cut-off values.
Relative levels of gene expression were first computed with GeneSpring 7.1 to obtain data sets of differentially regulated genes based on cut-off values of 5% error rate (p<0.05, determined by t-test with Benjamini and Hochberg Multiple Testing Correction).
Cut-off values for categorization of variables were based on cut-off values from the literature.
And finally, lack of MI might bias selection decisions based on cut-off values [ 6].
This filtering criterion based on cut-off values of fold-change provided a pool of candidate genes differentially expressed.
Alternatively, a statistical package can be used and the calculations based on cut-off values taking each of the full range of data values.
Another study observed a normal proxy-reported QoL in more than 90% of their sample based on cut-off values, yet this result was not based on inferential statistics [ 43].
If MI does not hold between two or more groups in a measure, estimates of mean differences between these groups [ 5], correlations with other constructs [ 3] or selection decisions based on cut-off values [ 6] may be biased.
For most sets of MetS criteria including the Adult Treatment Panel III (ATP-III)—MetS is classified based on cut-off values for each of the individual components [ 3, 7- 9].
One category was based on equal numbers of children exposed to different nitrate levels (0.25-2.08, 2.10-6.42, and 6.44-41.19 mg/L nitrate); the other was based on cut-off values of 10 and 25 mg/L nitrate.
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