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H and C assignments are based on COSY and HSQC experiments.
13,27,32 H and C assignments are based on COSY and HSQC experiments.
The resonance assignments were based on COSY, J-modulated, and HSQC 2D spectra.
H- and C-assignments were based on COSY and HSQC experiments.
Based on COSY correlations, two of the double bonds (C2 C3 and C4 C5) form a diene that by HMBC correlations, was further conjugated to carbonyl C1.
Initial analysis revealed the presence of a select highly mobile residue based on COSY data (26), and initial NMR-derived structures of E. coli ACP were improved using refinement against two structures (27).
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The major difference between them was that the keto group in 4 disappeared while one more methylene carbon (δC 28.4) appeared in 5. Based on 1H-1H correlationstiofs of H2-1 and H-3 with the methylene protons, it was elucidated that this menthylene was ascribable to C-2.
The assignments were based on DEPT, 1H-1H COSY, HSQC, and HMBC experiments Open image in new window Fig. 2 Key1H-1H COSY, HMBC and ROESY correlations of compound 1.
The assignments were based on DEPT, 1H-1H COSY, HSQC, and HMBC experiments.
The assignments were based on DEPT, 1H 1H COSY, HSQC, and HMBC experiments Open image in new window Fig. 2 Key 2D NMR correlations of saponaceolide R (2).
Likewise, C-9 was confirmed as a hydroxy substituted methine based on the 1H 1H COSY and HMBC correlations as shown in Fig. 2.
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