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QLT could have sufficed as a normalization procedure in its own right, but dendrograms (i.e. tree diagrams) based on correlation distances between gene expression profiles indicated that the addition of quantile projection normalization (QPN) [22] after QLT had a slight positive effect on the clustering of expression profiles into the correct experimental groups.
Significantly differentially transcribed genes were clustered using a hierarchical approach based on correlation distances.
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For clustering samples on the basis of their expression profile, we applied hierarchical clustering based on correlation distance and complete linkage.
Disease clustering was done in R (http://www.r-project.org) using the pvclust R package with "complete" setting for hierarchical clustering, based on correlation distance of R-scaled scores between genes and diseases, with 100 bootstrap replications.
Clustering of fertile and sterile lines in Figure 6C were performed based on correlation distance and complete linkage with normalized signal intensities of 24,577 probes using the R program.
b Hierarchical clustering of dataset 4 Evenness profiles was performed based on correlation-based distance and visualized using dendrograms.
Such variability makes identification based on correlation or other distance metrics impossible.
Yap et al. [16] generate the tetra-nucleotide usage pattern profile for each virus from which a distance matrix based on correlation coefficients is created.
For the MDS analysis, we constructed the distance matrix based on correlation scores among the samples.
Usually objects, units or cases are clustered based on sort of distance, whereas variables are clustered based on correlation coefficients with a goal to find optimal group [14].
For cluster analysis of the tissue expression profiles of the various ZNF genes the original relative quantitative RT-PCR values were used to calculate trees: The ZNF gene tree is based on Pearson correlation distances and the tissue tree on euclidean distances, both with single linkage, using R software http://www.r-project.org.org
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