Sentence examples for based on conserved segments from inspiring English sources

Our new measurement of sequence conservation calculates the sequence similarity based on conserved segments.

To show the benefit of measuring the sequence similarity based on conserved segments without considering divergent sequences in a region, we further examined the human regions with significant mouse BLAST hits.

Based on conserved chromosomal segments of the amphioxus and human genomes, Putnam et al. [ 53] reconstructed a total of 17 ancestral CLGs of which CLG3, CLG15 and CLG17 showed syntenic association with the α-containing human chromosome 16.

Quantitative RT-PCR for influenza was performed with primers based on conserved regions in the HA segment, H1N1-HA-275F (5'- GGGandTCCAGAGTGTGAATCACT-3') and H1N1-HA-375R (GCTCTCTTAGCTCCTCATAATCGATG-3') using a Stratagene MX3005P Real-Time PCR system.

The homologous primers for middle segments were designed based on conserved sequences of GPAT genes from some plants in NCBI GenBank (GPATF1, F2, R1, and R2, Table 1).

Sequences corresponding to each influenza segment were obtained by one-step RT-PCR using either a Qiagen One-Step RT-PCR kit (Qiagen, Valencia, CA) or an Invitrogen SuperScriptIII One-Step RT-PCR kit with High Fidelity Platinum Taq (Invitrogen, Carlsbad, CA), with primers designed based on conserved terminal and central regions of each segment (Table S3).

Based on conserved DNA sequences unique to the target organism, the results offer accuracy comparable to conventional tests.

Based on conserved motif of certain enzymes, chromosome walking provided a new method to identify functional enzymes [44].

Primers were designed based on conserved nucleotide sequences within the 52K gene.

We used degenerate primer pairs based on conserved amino acid sequence compared across multiple species.

Gene-specific primers were designed based on conserved regions (Table S2).