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Some sequences from completely sequenced genomes were re-annotated (Datasets S1 and S2), based on conserved protein motifs and gene structures of each family.
Genes more highly expressed in the pupal stage relative to other stages were largely unclassified based on conserved protein domains (Table 3).
Due to the multidrug resistance of the S. pneumoniae pathogen and the different geographic distributions of its serotypes, many studies are being conducted to develop effective formulations based on conserved protein antigens.
Numerous efforts have been made to obtain the sequences of insect OBPs [ 9, 12- 20] and CSPs [ 14, 21- 28] by direct cloning, which normally involves designing degenerate primers based on conserved protein sequences, amplifying the fragment and obtaining the full length sequences by Rapid Amplification of cDNA Ends (RACE).
These indels and SNPs were detected and genotyped according to Harel-Beja et al. (2010) [ 18] in genes associated with organic acid metabolism or transport (designated OAMG-organic acid melon genes) that were cloned by two methods: (1) from melon cDNA and gDNA by PCR using degenerate primers based on conserved protein sequences; (2) ICuGI database mining.
Overall, the average RPKM/protein, based on conserved protein annotation, across the five developmental stages of the mesocarp, were also abundant for those genes involved in glycolysis or the generation of pyruvate and subsequently fatty acid synthesis (Additional file 1: Table S3; Fig. 2c).
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In addition, they allow robust phylogenetic analysis based on conserved proteins of the oxidative phosphorylation system.
7, 23– 26 New approaches based on conserved proteins are in late-stage clinical trials.
In addition, progress has been made in serogroup B-specific vaccines based on conserved proteins and outer membrane vesicles.
A phylogenomic tree based on conserved proteins among all taxa used in this study showed O. polyrhachis-furcata to be closely related to O. sinensis which is from the same family (Ophiocordycipitaceae) and clustered with other fungi from the order Hypocreales including other entomopathogenic fungi (Fig. 1).
AAI is calculated based on conserved protein-coding genes between a pair of genomes which are determined by whole-genome pairwise sequence comparisons using the BLAST algorithm [ 34].
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based on antigen protein
based on conserved phosphorylation
based on conserved degradation
based on conserved core
based on conserved motif
based on total protein
based on conserved synteny
based on conserved flanking
based on conserved co-expression
based on conserved ortholog
based on conserved exon
based on conserved sequence
based on conserved binding
based on lyophilized protein
based on conserved signature
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