Sentence examples for based on conserved motif from inspiring English sources
Based on conserved motif of certain enzymes, chromosome walking provided a new method to identify functional enzymes [44].
So far, the genome of P. moravensis has not been published, and thus potential lipase genes were cloned using primers based on conserved motifs.
Post genome sequencing projects have enabled relatively easy identification of particular gene families based on conserved signature motifs and sequence similarity.
However, computational identification of RNA substrates based on conserved binding motifs is not straightforward because in addition to the sequence context structural features of the RNA are relevant.
The conserved O. tauri components required for GSK3-mediated S6K regulation are shown in Figure 5, overlaid with the mammalian interactions based on conserved phosphorylation motifs and binding sites.
SOX genes belong to the High Mobility Group (HMG) superfamily and encode transcription factors which can be identified based on a conserved motif within the HMG domain, RPMNAFMVW [ 5].
In this study, we designed a universal degenerate primer pair based on highly conserved motifs at the N-terminus of the small subunit and C-terminus of the large subunit.
The 36 WRKY domains of group II each contained one WRKYGQK motif and a C2C2-type zinc finger motif C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-H C-X5-C-X23-H-X1-Hed in the WRKY domand.
One complication is that TFs from the same structural family often bind to highly similar DNA target sequences (Luscombe et al. 2000), and it can be difficult to separate sites for different TFs based on the conserved motifs alone.
Author's response: See reply to reviewer 1 – we have removed these statements that were based, not on phylogenetic trees with bootstrap support (rare at these evolutionary distances), but on scores from homology searches and on conserved motifs.
