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The sequence contigs were compared with corresponding regions in trypanosomatids (based on conserved gene order, where this existed), to examine gene content and the conservation of gene order (i.e., colinearity) and, therefore, the potential for using trypanosomatid genome sequences as scaffolds to assist assembly and annotation of the B. saltans sequence.
The syntenic blocks can also be observed in the graphical representation of the synteny scores (Figure 9) based on conserved gene order (the scoring system disregards gene direction).
The tree based on conserved gene pairs appeared to combine phylogenetic information with major effects of horizontal transfer of operons.
The complete proteobacterial and Gram-positive bacterial clusters were recovered in this tree as well as the unexpected grouping of chlamydia with spirochete noticed above in the tree based on conserved gene pairs (Fig. 4, 5).
On the other hand, the assumption that CD14+CD16+ human monocytes are equivalents of murine Ly6Clo monocytes is primarily based on conserved gene and protein profiles between these subsets [ 16], but not on functional assays.
The trees based on presence-absence of genomes in orthologous clusters and the trees based on conserved gene pairs appear to be strongly affected by gene loss and horizontal gene transfer.
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These results show clear distinction between the three closely related species based on conserved genes, and V. metoecus is a much closer relative to V. cholerae than V. mimicus.
Trees based on conserved genes, such as wbmA, were similar to the genome-wide SNP tree and the previous multi-locus sequencing typing (MLST) tree [ 1], with B. bronchiseptica complex I and IV strains clustering separately, for example.
A maximum-likelihood inference-based dendrogram was used to generate phylogenetic trees based on conserved orf288 genes in mitochondrial genomes.
Phylogenetic analysis based on conserved core genes and complete genome sequence of STIV with other virus genomes was performed.
AAI is calculated based on conserved protein-coding genes between a pair of genomes which are determined by whole-genome pairwise sequence comparisons using the BLAST algorithm [ 34].
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based on conserved degradation
based on conserved motif
based on conserved domain
based on conserved flanking
based on conserved ortholog
based on conserved co-expression
based on conserved codon
based on conserved adenovirus
based on conserved exon
based on conserved influenza
based on conserved binding
based on conserved signature
based on conserved protein
based on conserved synteny
based on conserved genomic
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