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The Locus finder tab accesses identical microsatellite loci across species based on conserved flanking sequences (approximately 25 bp long) on either side of the polymorphic loci.
In another case (PHYC), nucleotide divergence at a commonly used primer-binding site prevented the characterization of the hinge region by the typical strategy of using primers based on conserved flanking peptides HYPATDIP and PFPLRYAC.
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A 1339 bp DNA fragment of the putative oriC region from the rifamycin SV producer Amycolatopsis mediterranei U32 was cloned by PCR amplification employing primers designed based on the conserved flanking genes of dnaA and dnaN.
The phylogenetic reconstruction was based on conserved domains.
When no syntenic gene was identified, a predicted location was generated based on syntenically conserved flanker genes.
Additionally, algorithms were implemented for finding loci across species, based on the presence of identical simple sequence repeats (SSRs) with the same or varying frequencies of repeat units but conserved flanking regions.
A series of highly conserved flanking cysteines (Cys-53, Cys-77, and Cys-83) were mutated to alanine, but none of these mutations showed any effect on either enzyme activity.
Other conserved flanking genes are translation-associated.
The sequence logos confirmed that evolutionarily conserved phosphosites also contained highly conserved flanking regions.
Conserved residues are shaded by different intensities based on conservation level in the alignment.
Primers were designed based on highly conserved iridoviral sequences.
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