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We also noted that many of the mAbs grouped in terms of V(D J rearrangement showed signs of clonal expansion based on conserved codon usage (Fig. 2).
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The codon site 213 was an significant residue (p-value 0.003) based on changing properties in secondary structure, along with codon site 216 (p-value 0.042) based on conserved properties in polarity and iso-electric point.
Both codon sites selected in SRS6 were based on conserved properties, and not predicted to constitute the active site (Additional file 5).
The phylogenetic reconstruction was based on conserved domains.
Despite the fact that the comparison was based on 200 conserved gene homologs, the codon preferences for D. melanogaster are consistent with those reported by Vicario et al. [ 27].
Conserved residues are shaded by different intensities based on conservation level in the alignment.
Primers were designed based on highly conserved iridoviral sequences.
Rare codons for Asp and Pro were replaced by more frequently used ones based on E. coli codon usage.
In B. subtilis the proportion of the tRNAs pairing to more than one codon is calculated based on the codon usage ratio.
The codons for sTfR were optimized using human preferred codons based on Codon Usage Database (Kazusa DNA Research Institute, Japan).
COrrespondence Analysis of codon usage (COA) was calculated based on codon usage as well as Relative Synonymous Codon Usage (RSCU).
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