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The part dedicated to influenza viruses, presented here, was based on consensus sequences specific for one gene.
The part dedicated to influenza viruses was based on consensus sequences specific for one gene generated from global alignments of a large number of influenza virus sequences available in databanks.
Two sets of primers that showed high specificity for the target arsC gene were designed based on consensus sequences from 13 bacterial species.
Given the sequence heterogeneity within env, our use of subject-specific primer sets with degeneracy may represent a "best-case" scenario for template amplification; the use of primers based on consensus sequences could result in less efficient amplification.
The 20 amino acid peptides with 10-amino acid overlaps between sequential peptides were based on consensus sequences of clades A, B, C and D Gag protein and Clade B Nef, Tat, Vif, Rev, Vpr, Vpu and Pol proteins.
Studies that demonstrated inverse correlations between IFN-γ responses and plasma viral loads related this to a broader HIV-1 epitope repertoire [9], [42] which in this study could have been limited by the use of peptides based on consensus sequences instead of autologous sequences.
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Considering the high conformation similarities in contrast to huge differences of OPH activity between AbOPH, OPHC2 and MPH, a semi-rational design based on consensus sequence approach was performed to identify the key residues that depress the OPH activity of AbOPH.
Unlike most existing methods based on consensus sequence profiles, our approach correctly considers the phylogenetic context as it aligns new sequences against inferred ancestors.
The evolutionary trees were constructed based on consensus sequence by the MEGA4.0 [ 38] program with neighbor-joining [ 50] phylogeny using the p-distances model.
Promoter regions were manually identified upstream of transcription start sites identified through directional RNA-Seq data based on consensus sequence similarity or based on published data and annotated accordingly.
AluScan implemented with just a small number of PCR primers based on consensus Alu sequences provides a multiplex method for genome-wide sequence analysis.
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