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The SSCP technique is based on conformational differences of single stranded DNA fragments that can be detected as mobility shifts in non-denaturing polyacryilamide gel electrophoresis [ 15].
These species-specific phenotypes can be due to different functions of eIF5A(Dhp50) and eIF5A Hyp50) in single- versus multicellular organisms and might be based on conformational differences between eIF5A(Dhp50) and eIF5A Hyp50) that lead to interactions with pathways specific to phylogenetically higher organisms.
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Substrates with higher molecular weights may influence the mass transport to the immobilized enzyme, but not alter the observed difference in immobilization methods since it is based on conformational effects.
Therefore, APC differs from AGC, which has a regulatory mechanism based on conformational changes within a dimeric regulatory domain.
During Data Pre-Processing descriptors based on conformational ensembles are computed and stored in a database.
Furthermore, based on conformational changes observed for Uba2 at earlier steps in the Sumo activation cycle, it seems that the Uba2 cysteine domain may also undergo conformational changes to meet the Ubc9 cysteine for Sumo transfer [20].
Cumulating experimental data conflict with mechanisms of disorder-to-order transitions solely based on conformational selection.
In addition, immunoassays based on conformational antibodies have been successfully used to screen DAACPs at the tissue and cellular level.
The switching mechanism on electrically switchable oligopeptide surfaces is based on conformational changes between collapsed ("OFF" state) and fully extended ("ON" state) oligopeptide structures.
Two groups disagree on the possibility that the two proteins could have similar switch mechanisms based on conformational rearrangements [ 39, 40].
This difference is explained based on different conformational states of the adsorbed chains, in particular by loop formation of the triblock copolymers.
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