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It is indeed often necessary to assay several amiRNAs, the sequences of which are based on computational predictions, to find one which effectively triggers knock down of a target gene (Warthmann et al. 2008).
Based on computational predictions from both miRBase and TargetScan, there are a total of 25 predicted target mRNAs for miR-29 within our data displaying decreased expression.
It appears that for most basic research applications, cleavage activity can be maximized and off-targets minimized by carefully choosing guide RNAs based on computational predictions.
GO (http://www.geneontology.org) [22] annotations are partially based on computational predictions whereas the HPP list [23] is based on a combination of experimental methodologies.
In addition to data from literature, we also include additional information based on computational predictions.
In particular, the added coordinates for missing residues and atoms are based on computational predictions and may not be reliable.
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Therefore, we considered PDCD4 to be a miR-181b target based on computational prediction and the inverse correlation between miR-181b and PDCD4 protein levels in human CRC tissues.
The information retrieved was based on computational prediction and/or experimental documentation compiled in SUBA; the Arabidopsis SUBcellular localization database [28].
For proteins not having annotated subcellular information, their subcellular location assignments are based on computational prediction.
Many bovine proteins are only partially annotated or are based on computational prediction.
We have recently presented an inventory of spliceosomal RNAs based on computational prediction from genomic sequences [ 8].
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based on computational models
based on computer predictions
based on optimistic predictions
based on simple predictions
based on computational results
based on suboptimal predictions
based on modelling predictions
based on computational approaches
based on fragile predictions
based on computational simulations
based on computational experiments
based on nominal predictions
based on computational algorithms
based on hydropathy predictions
based on structural predictions
based on present predictions
based on reliable predictions
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