The method is based on competition between binding sites, and measures relative sequence-specific DNA-binding affinity of a TF.
The IC50 value (half maximal inhibitory concentration) was calculated based on competition nonlinear binding curve using GraphPad Prism software Version 7.03.
Indeed, previous DISC models did not consider DED chains upon model construction and were based solely on competition binding mechanisms, between procaspase-8 and c-FLIP for binding to FADD at the DISC.
These kits are based on competition for a cAMP binding site on an antibody or cAMP binding protein and as such have a limited dynamic range.
This can lead to problems in the development of small molecule inhibitors based on competition at the ATP-binding site of a kinase, as the ATP concentration in vivo may differ greatly from that used in vitro.
ITC can measure heat change directly during the binding interaction and therefore provides direct access to thermodynamic parameters and Kd [ 54], while the Lipidex assay is more likely to have imprecise Kd values since it is based on competition and not directly measuring the binding event [ 25, 54].
EGFR concentrations in crude membranes were analysed by a ligand-binding assay based on competition between I-EGF and unlabelled EGF (Santini et al, 1991).
We take a prokaryotic repression model based on competition between repressors and RNA polymerases, where DNA-bound repressors prevent RNA polymerases from reaching their binding sites and initiating transcription.
The principle of the rat LH assay is based on competition between the LH of the rat sample and a I-labeled rat LH tracer for binding to a highly specific rabbit polyclonal antibody.
The subsequent solid-phase enzyme immunoassay is based on competition between SAH in the sample and immobilized SAH bound to the walls of the microtiter plate for binding sites on a monoclonal anti-SAH antibody.
