Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix, and the procedures were performed according to the manufacturer's instructions.
Similar results were obtained with a method based on column-switching recycling size-exclusion chromatography, microenzymatic online digestion, and LC-MS [ 101].
Methods based on anion exchange column chromatography of LV vectors pseudotyped with VSV-G [ 17, 18] or the baculovirus GP64 glycoprotein were previously described [ 19].
A methodology based on multisyringe chromatography with a monolithic column was developed to determine three sulphonated azo textile dyes: Acid Yellow 23, Acid Yellow 9 and Acid Red 97.
are based on enzymatic and chromatography techniques (e.g., gas chromatography or high-performance liquid chromatography (HPLC)) [ 5– 8].
Serum Met, Ser, Tau, and Gly were measured using an automatic amino acid analyzer (Hitachi L-8800, Tokyo, Japan) based on cation-exchange chromatography and post-column reaction with ninhydrin reagent.
The fractions were compiled based on thin layer chromatography (TLC) profile to obtain five main fractions, which were again subjected to column chromatography (C.C).
In this work a bioanalytical method based on coupled column liquid chromatography tandem mass spectrometry (LC MS/MS) is presented for quantification of a peptide drug, FE 202158, under clinical development.
Thirdly, an additional purification step, possibly based on immuno-affinity chromatography of the FLAG-tag (Einhauer and Jungbauer, 2001), might be required to remove endogenous C. albicans proteins that co-purify with GR reporters on the nickel columns.
In present work we describe a pDNA purification process designed on two different CIM monolithic columns, based on anion-exchange (AEX) chromatography and hydrophobic interaction chromatography (HIC) chemistry.
