Sentence examples for based on cloning and from inspiring English sources

Lcc genes from Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 were characterized based on cloning and sequencing analysis.

PCR products were checked by 10% PAGE electrophoresis to separate the target DNA bands and which were following confirmed based on cloning and sequencing.

For user, defined number of times, the B-Cell is shown to the antigens from all groups and fitness value is calculated; population pruning is done after that, two affinity maturations based on cloning and mutations are performed and some random selected antigens from the same group are inserted to the set.

A number of studies are based on cloning and sequencing [4], [5], [6], [7].

Here we introduce an exact non-neutral model of vegetation dynamics, based on cloning and seed dispersal, which yields closed-form characterizations of beta-diversity.

We found that V. longisporum strains contained two MAT1-1 idiomorphs based on cloning and DNA sequencing (Table S1), and failed to detect MAT1-2 usidiomorph-specificific PCR screens (Figure S4).

Here, based on cloning sequencing and polymerase chain reaction-restriction fragment length polymorphisms, we analyzed polymorphisms in the first hypervariable region and coding regions of mitochondrial DNA of 19 human bone remains which were excavated from a tomb near the Terra Cotta Warriors and dated some 2,200 years before present.

This paper is the first analysis of the dog genome using massively parallel next generation sequencing techniques, as opposed to the previously published boxer reference genome, which was based on cloning in bacterial cells, Sanger sequencing and whole genome shot-gun assembly.

Analyzing development-related miRNAs expression at 14 developmental stages based on clone-sampling and stem-loop RT PCR, we discovered an unusual abundance of 33 sequences representing 12 different miRNAs and sharply fluctuated expression of miRNAs at larva-molting stage.

The analyses of ca. 250,000 sequence reads revealed that the number of detected Operational Taxonomic Units (OTUs) far exceeded previous richness estimates from the same sites based on clone libraries and Sanger sequencing.

Furthermore, the number of detected OTUs far exceeded previous richness estimates from the same sites based on clone libraries and Sanger sequencing, even when tag sequences were clustered at up to ten nucleotide differences (reflecting ca. 8% sequence similarity).