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In place of an enterokinase recognition site, a polyhistidine tag linked to an autocatalyzed site based on cleavage specificity of the serine protease flanking on the 5′-end of Tm-5 clone sequence was engineered before protein expression in E. coli system.
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Based on the cleavage specificity of the proteinase subunits, Benoist et al. suggested a role in degrading cell wall proteins such as extensins [ 7].
In the first digestion step (step 7), the linkage between the first and second disaccharides on the chain is assigned a probability of cleavage based on the specificity of the particular lyase [30], [37] [42] (Figure 1 and Materials and Methods).
The detection and quantification of known SNPs are primarily based on the specificity of target hybridization and enzyme discriminations, such as specific enzymatic cleavage, and single base primer extension.
Recommendations include: (1) Specificity: based on specificity to a particular small RNA population.
Among others, one valuable approach proposed for SNP discovery is based on base-specific cleavage and mass spectrometry [ 1- 3].
One valuable approach proposed for SNP discovery is based on base-specific cleavage and mass spectrometry.
Based on the substrate specificity [Fig. 3(C ], the cleavage of RNA by GCN4 LZ and rLZ may involve elements of an RNase T1-like mechanism.
Instead, she customized her own approach to offering praise based on detail and specificity — which were important values to her.
This assay is based on the cleavage activity of the light chain.
The specificity of caspase-1 for cleavage of pro-IL-1 β is suggested to be due to the labile nature of its activity, while it appears a rather promiscuous enzyme based on substrate cleavage profiles.
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