Probe 1 was based on cleavage of ester and intramolecular charge transfer (ICT) effect, which showed fast-response property toward hNQO1 for real-time enzymatic assay in vitro.
Probe 2 was based on cleavage of amide and photo-induced energy transfer (PET) effect, which was stable, cell-membrance-permeable, low cytotoxic, and highly selective and sensitive for hNQO1.
The assay is based on cleavage of the chromogenic substrates, DEVD-pNA, IETD-pNA and LEHD-pNA, by caspases-3, -8 and -9, respectively.
General MMP activity was measured by means of a fluorimetric assay based on cleavage of the fluorogenic peptide substrate FS-6 (Calbiochem, San Diego, CA, USA).
NSCLC cell proliferation was assessed by using an assay based on cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases (Roche, Indianapolis, IN) as reported earlier [ 22].
After experimental treatments, cell proliferation was measured by colorimetric assay based on cleavage of a tetrazolium salt 3- 4,5-dimethylthiazol-2-yl -5- 3-carboxymethoxyphenyl -2- 4-sulfophenyl -2H-tetrazolium (MTS) by mitochondrial dehydrogenase enzyme present in proliferating cells (Cell titer 96 - Promega, Madison, WI, USA).
In place of an enterokinase recognition site, a polyhistidine tag linked to an autocatalyzed site based on cleavage specificity of the serine protease flanking on the 5′-end of Tm-5 clone sequence was engineered before protein expression in E. coli system.
Fertilization rate was based on cleavage evaluation of 50 embryos per group as described previously [ 57, 58].
In this work, a sensitive, specific, and reverse transcription-free approach for quantification of mRNA based on cleavage-mediated isothermal exponential amplification reaction (IEXPAR) is established.
LuloMVP1, LuloMVP2 and LuloMVP5 are of nearly equal mature molecular weight of 21 kDa based on the cleavage of the predicted signal peptide present in all of the microvillar proteins and LuloMVP3 and LuloMVP4 are slightly larger; around 23 kDa.
Chemical methods are based on the cleavage of 1 4 glycosidic bonds of the cellulose chains and an isolation of the cellulose nanocrystals eliminating the amorphous cellulose part [22].
