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RT-PCR products were cloned, sequenced, and assigned to genomic HML-2 loci based on characteristic nucleotide differences between loci.
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Generated HML-2 cDNA sequences were assigned to genomic loci based on characteristic (private) nucleotide differences distinguishing the various HML-2 loci in the human genome from each other.
Apoptosis was assessed based on characteristic DNA staining.
We considered that the most likely factors to bind to the PPARA nuclear receptor half-site were estrogen related receptor (ERR)α and ERRγ, based on the characteristic nucleotide sequence [32], [33], on known similarities in tissue expression between ERR and PPARα [34] [36], and on ERR regulatory activities for genes downstream of PPARα [28], [35], [36].
Adaptations were made based on characteristics of the target population.
Other methods based on nucleotide statistics are sensitive to nucleotide periodicity in CDS.
A strategy has been proposed by Daffonchio et al. [123] for the identification of near neighbours of B. anthracis based on single nucleotide polymorphism (SNP) in the 16S 23S rDNA ITS containing tRNA genes, characteristic of B. anthracis.
Based on their nucleotide sequence, miRNAs are grouped into distinct families, each family comprising one or more members.
Type M was further divided into 33 subtypes based on single nucleotide polymorphisms, single nucleotide deletion (V12), double nucleotide deletion (V19) and single nucleotide insertion (V20) (Tables 3, 4).
Based on the nucleotide variations, a total of 17 single nucleotide polymorphisms (SNPs) and five insertion/deletions (indels) of Gn1a were detected in O. sativa.
Therefore genotyping based on single nucleotide polymorphisms (SNPs) can indicate their origins, spread and phylogeny.
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