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Fig. 8 A median longitudinal view of a small lateral root visualized under UV based on cell wall autofluorescence using a two-photon laser scanning microscope.
To confirm that the growth phenotype observed in LOC-MyoD/Id2-CAT-transformed cells is due to Och1p activity, Alexa488-modified WGA lectin was employed to fluorescently label yeast based on cell wall composition.
Classical approaches for the study and identification of CWPs are based on cell wall purification, protein extraction, separation by electrophoresis and identification of proteins by mass spectrometry.
Based on cell wall compositions, ten wheat accessions and three rice mutants were classified into three distinct groups each with three typical pairs.
In addition, the PLS model regression coefficients resembled cell wall spectral peaks (Additional file 9) indicating that the model was based on cell wall chemistry.
Anatomically, based on cell wall type, vegetative branching pattern, root development, and inflorescence branching Brachypodium is a typical grass [ 12, 16].
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Based on cell-wall composition, we selected ten wheat accessions from various genotypes and three rice mutants with different phenotypes, which were divided into three distinct groups (I, II and III) with nine pairs.
However, their application under dc field limits the distinctions to those based solely on cell wall permeability, rather than including distinctions based on capacitance and permittivity effects.
Based on the cell wall composition, the nematode must produce a specific set of CWDEs, to degrade a host specific cell wall for successful entry into a plant species.
A modified version of the PrepEase Genomic DNA Isolation Kit (Affymetrix, 78855 1 KT) based on enzymatic cell wall digestion was used for the DNA isolation from yeast cells.
In order to test the efficiency of this method for quantifying anaerobic rumen fungi, it has been attempted to evaluate this method in in vitro conditions by comparing with an assay based on measuring cell wall chitin.
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