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By sampling tissue based on cell populations that express the enzyme, we avoided dilution of active enzyme by a variable amount of tissue that could potentially produce biased results and were thus able to assess enzymatic activity in a more precise manner than previously.
This allowed the definition of the major gate for NC based on cell populations from the mixed IVD cell type.
This review focuses on recent advances in the cellular and molecular biology of skeletal muscle regeneration based on cell populations described to play a role in this process.
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The measurements in the Hathaway [ 31] article are based on cell population dynamics of histone modifications measured in ChIP experiments, whereas our simulations concern histone modification pattern formation at the level of a single gene.
For example, several groups have shown that cells belonging to the basal population (based on cell surface markers) have the potential to generate all mammary epithelial cell types when transplanted by themselves in cleared mammary fat pads, and these cells are referred to as mammary stem cells [ 7- 9].
By sorting peripheral blood from healthy donors based on cell surface markers, we obtained purified populations of CD4+ T-cells, CD8+ T-cells, and B-cells.
All these methods introduce assumptions and biases that might result in selective loss of certain populations of cells based on cell size, surface antigen expression or biophysical properties.
Working at the level of individual cells is important due to cell-cell variability [1], [2], [3], [4], which is masked in assays based on averaging cell populations.
Since the enrichment of progenitors proportion could enhance the effectiveness of treatments based on these cell populations, the interest on the effect of culture conditions on the TSPCs is growing.
DOI: http://dx.doi.org/10.7554/eLife.03104.004 10.7554/eLiFigure04.005 Figure 3. Adult born granule cells (3 10 week old) can be divided into two distinct populations based on cell-to-cell differences in input output transformation.
The apoptotic cell population was further assessed by flow cytometry after staining cells with Annexin V-FITC and 7-AAD, based on the cell population in the top right (late stage of apoptosis) and the bottom right quadrants (early stage of apoptosis).
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