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Assays were based on cell permeabilization, staining with phosphorylation-specific antibodies and flow cytometry.
They were based on cell permeabilization and staining with phosphorylation-specific antibodies.
Here, new direct assays are introduced, which are based on cell permeabilization, Bax phosphorylation-specific antibodies and flow cytometry.
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The membrane localization of ERs is based on preventing cell permeabilization by careful optimization of fixation and handling techniques for a given cell type.
Their killing action can be based on membrane permeabilization and cell lysis, as well as their interactions with cytoplasmic targets.
Drosomycin lyses fungal hyphae suggesting a mode-of-action based on membrane permeabilization and pore formation in susceptible fungi (Fehlbaum et al., 1994).
Cell death was quantified based on plasma membrane permeabilization.
The first allows the monitoring of effects on trafficking pathways by biochemical assays, and is based on plasma membrane permeabilization using the bacterial toxin streptolysin-O.
Also, we showed that MAdCAM-1 was present on the external endothelial cell surface based on immunostaining of the cell surface without prior permeabilization (see Figure 2C).
Cells were gated appropriately based on forward scatter and side scatter and cell permeabilization was defined by PI-positive staining.
The methodology is based on formaldehyde crosslinking of cells, followed by permeabilization, antibody staining, flow sorting, reversal of the crosslinks, and recovery of the RNA.
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