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In breast tumors, we confirmed that this panel of analytes can be reproducibly measured at levels above assay background and over a dynamic range consistent with expectation based on cell line models.
Yet, there is still evidence that upregulation of FGFR1 transcription may have a role, based on cell line findings where casodex was applied for 48 h.
In addition to simple co-expression, actual cross-talk between ErbB2 and AR pathways has been suggested based on cell line studies in breast [ 5, 6].
Analysis of additional samples based on cell line availability resulted in validation of a MAPKAPK5- ACAD10 fusion transcript in two probands.
AXIN2 is amplified and over-expressed in CRC tissues; it also shows a CRC lineage-specific dependency based on cell line data from the Achilles project (Fig. 3e).
The microRNA assays selected for the arrays were for 46 microRNAs based on cell line profiling results (Supplementary Table S1), and ath-miR-159a, which was a spike-in control during plasma/serum extraction.
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This may mean that many stem-cell treatments created by U.S. researchers would be based on cell lines created in India, Australia or Sweden.
Most assays that exist today are based on cell lines often over-expressing the substrate.
Most data presented for subcellular fractions of RNA are based on cell lines [ 20, 23].
Authors' response: Based on our experience we suggest using at least 3 replicates for studies based on cell lines.
Most of the commonly available in vitro assays of erythropoiesis are based on cell lines, such as murine MEL or human K562, HEL, and UT-7 cell lines.
More suggestions(15)
based on yield line
based on cell density
based on cell permeabilization
based on cell morphology
based on cell tracking
based on cell aggregation
based on growth line
based on cell overlapping
based on cell seeding
based on cell biology
based on intensity line
based on cell lineage
based on cell proliferation
based on cell sorting
based on cell number
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