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Current in vitro methods require cells to self-organize into hollow morphologies; we explored an alternative strategy based on cell growth in predefined, spherical scaffolds.
Complementation was evaluated based on cell growth on SC agar medium lacking AAAs (SC-AAA), since TKLs are involved in AAA biosynthesis via effects on the supply of erythrose-4-phosphate.
Several studies have developed cell consortium model based on cell growth, fermentation kinetic model and dynamic flux balance model to study the capability of co-culture system and to optimize cell growth and mixed sugar fermentation performance by co-culture (Unrean and Srienc 2010; Unrean and Khajeeram 2015; Hanly and Henson 2013).
The tolerances of E. coli cells containing these mutants were enhanced 2 to 50-fold, based on cell growth tests using different concentrations of alcohols or acetate, and enhanced 10 to 100-fold based on ethanol or butanol shock experiments.
The IC50 value was defined as the concentration of gefitinib needed for a 50% reduction in absorbance (560 nm) based on cell growth curves.
In addition to molecular characterization (p53, HER2), the cell lines were characterized based on cell growth characteristics including spheroid growth, migration potential, and anchorage independence.
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M. aeruginosa photosynthetic activity was found to be the most sensitive endpoint to enrofloxacin (EC50 72 h =0.02 mg/L), followed by growth inhibition based on cell number.
Growth inhibition based on cell number, bio-volume, chlorophyll-a concentration as well as photosynthetic activity were used as evaluation endpoints in the single-species tests, while growth inhibition based on measured chlorophyll-a was primarily used in the competition experiment.
This rapid extension growth is based on cell elongation and accelerated cell division, which is attended by the consumption of energy and solutes[26].
According to the growth curve based on cell counting and in vivo Chl a fluorescence with and without addition of DCMU (3- 3,4-dichlorophenyl -1,1-dimethylurea), the cells divided once a day under these conditions.
Effects of IFN-α on cell growth and cell-cycle distribution were evaluated based on cell numbers and flow cytometry.
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