In addition to molecular characterization (p53, HER2), the cell lines were characterized based on cell growth characteristics including spheroid growth, migration potential, and anchorage independence.
At this stage, the gonads containing both Gc and Cn were categorized as indifferent gonads, because male- and female-specific Gc of fishes are difficult to differentiate solely based on cell morphological characteristics.
Importantly, SR and IR cells were indistinguishable from each other based on cell cycle distribution/proliferation characteristics.
Based on characteristic cell shape, size and their relative locations, cochlear cells in the sensory epithelial, lateral wall and stria vascularis are clearly identifiable in this live tissue preparation (various cell types are marked in Fig. 1,A&B).
The images were scaled and the following parameters were measured for the NP: 1) proportion of NP surface area and pericellular matrix consisting of NCs, which were identified based on morphologic characteristics (cell size, intracytoplasmic vesicles, typical NC clusters) [ 16, 36], and 2) proportion of NP surface area consisting of CLCs and fibrocartilaginous matrix.
Microfluidic isolation can be generally divided into two broad categories of enrichment modalities, either isolation based on the cell physical characteristics (e.g., size and density) or cell biochemistry (e.g., antigen expressions).
The state of the cycle was defined based on the characteristic cell content in the fresh lavage (Axiovert 40C, Carl Zeiss, Jena, Germany; magnification × 200) and stained (Axio Observer.A1, Carl Zeiss; magnification × 200).
Based on the cell-fusion characteristics of muscle cells, we have created a Cre/Lox-β-galactosidase (Cre-Lox) system [16] to specifically tag differentiated multinuclear myofibers, as well as mononuclear cells released from these tagged multinuclear myofibers via the in vivo dedifferentiation of the skeletal muscle after injury.
Next, analysis software was trained using ten representative original multispectral images to segment the different tissue regions (B cell follicle and stromal tissue (red pulp in spleen or lamina propria in appendix)) based on a combination of parameters including cell morphology and specific staining (Fig. 4g) and individual cells based on nuclear characteristics (Fig. 4h).
Tumor-initiating activity cannot be improved by sorting cells based on intrinsic characteristics and cannot be prospectively determined based on the environment in which the cells reside.
