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Results are expressed as picograms of metabolite per milligram of protein, based on calibration lines constructed with commercially available eicosanoid standards (Cayman Chemicals).
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Fig. 4 Divergent quantitative diagnosis based on calibration.
Quantification was based on calibration curves drawn using purified proteins.
Quantification of compounds was based on calibration curves constructed from respective standards.
LOD and LOQ calculations were based on calibration curves [ 111].
Quantification was based on calibration curves established using standard glucose.
Therefore, we also assessed model fit based on calibration plots.
In line with other studies (33, 35– 38), we calculated objectively assessed MVPA using the threshold of ≥2,020 counts/minute, but this threshold was based on calibration studies primarily carried out under laboratory conditions, not in free-living conditions (34).
Quantification was achieved based on calibration curves obtained using PCB standards.
Therefore identification of peaks based on calibration mixtures (starting from pure compounds) is necessary.
d Amount calculated based on calibration curve obtained with NS5 Standard NY1999 (column 1).
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