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The objectives of this study were to isolate, sequence, and characterize the genomic clone of peanut allergen, Ara h 3. A peanut genomic library was screened, using two [32P] end-labeled oligonucleotide probes designed based on cDNA sequences of Ara h 3 and Ara h 4. Four positives lambda FIX II clones were obtained after four rounds of screenings.
The first widely used microarrays were based on cDNA sequences [1].
Primers were designed based on cDNA sequences for M. sexta retrieved from genebank: Bbx (DQ080209), InR (FJ169464) and actin (L13764).
Although pedigree information has been used successfully to identify DRB haplotypes based on cDNA sequences from the red deer [27], few (if any) pedigree records are available for wild and semi-wild animals.
Amplimers were synthesized based on cDNA sequences from GenBank (2004).
Nevertheless, all these "mutant" HyPRPs have been found based on cDNA sequences, indicating, at least, their active transcription in vivo.
Similar(43)
Thus the correspondence between a well-annotated cDNA clone set and an mRNA transcript database based on cDNA sequence was no greater than the correspondence between either resource and our length dataset.
The gk367 genomic lesion results in the deletion of 92 AAs based on cDNA sequencing.
Specific primers were synthesized for genomic DNA amplification based on cDNA sequence.
The stringency of criteria for selection of sRNA candidate regions based on cDNA sequencing data has a great effect on the number of candidates derived from this approach.
However, developing microarray platforms for A. fumigatus has already been made outdated by the advent of new technologies based on cDNA sequencing.
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