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The 5 herds that were BHV-1 negative based on bulk milk testing were also free from seropositive individuals among the lactating cows (data not shown).
The importance of this has also previously been noted by Agren [ 5] and is highlighted in the case of BTV-8 detection in cattle in Norway, based on bulk milk testing rather than clinical signs [ 6].
* Sera with CODs ≥ 0.20 were interpreted as seropositive ** i.e. mean prevalence within the eleven herds All eleven herds were BVDV positive based on bulk milk testing (mean COD: 0.13; range: 0.05 0.23) and 6 out of 11 were BHV-1 positive (mean COD: 0.20; range: 0 0.56).
In countries with large dairy herds and with low BVDV prevalence (e.g. Denmark), the SVANOVIR could be preferred for an early warning surveillance system based on bulk milk testing, because a lower prevalence of seropositive milking cows can be detected (compared to the situation where the Danish blocking ELISA is used).
Currently, the decision criteria used by the Danish Cattle Federation to consider a herd as positive, based on bulk milk testing, is an increase in the blocking percentage to 50%% [ 3, 5, 10] and/or two consecutive bulk milk samples ≥ 20%%.
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More than 90% of U.S. dairy herds sampled were infected with C. burnetii based on bulk tank milk testing over a 3-year period.
A study based on bulk tank milk samples from 100 randomly selected dairy herds was performed to estimate the prevalence and geographical distribution of antibody positive dairy herds.
The prevalence of C. burnetii antibody positive Danish dairy herds in 2008 was 59% [[ 3]] based on bulk tank milk samples (BTM) from 100 randomly selected herds.
A study based on bulk tank milk samples from 120 randomly selected dairy cattle herds was conducted to estimate the prevalence of Coxiella burnetii seropositive dairy herds, to describe the geographical distribution, and to identify risk factors.
Many methods used in cell biology are based on bulk measurements on large cell populations.
Clinical surveillance of brucellosis is complemented by active surveillance, which consists of annual serological tests of each herd based on either bulk milk samples from dairy cattle or serum samples from 20% of beef cattle over 24 months old.
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