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Cell speed was calculated based on blinded observers tracking 75 cells per condition and using customized semi-automated program developed with MATLAB version 2008 (Natick, MA, USA).
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Based on blinded reviewer assessments, 67% of subjects showed improvement in appearance at 180 days.
Histological assessments were performed by one blinded observer (YT).
However, studies based on human observers are resource-demanding and involve a significant variability of intra-observer and interobserver performance.
In addition, the structure of the proposed observers is based on a discrete observer and a continuous observer on interaction.
The reliability coefficient was 0.98 based on 39 blinded quality control split samples.
The design is based on asymptotic observer.
Tissue sections were stained with Alcian blue/Orange G and graded by two blinded observers based on the scoring system developed by Chambers et al. [ 7].
Tissue sections were stained with Safranin O and graded by two blinded observers based on the scoring system developed by Chambers et al. [ 18].
The evaluation is based on an independent blinded peer review method and a formal classification of discrepancies.
The findings, reported in JAMA, are based on a randomized, blinded, placebo-controlled intervention among nearly 15,000 U.S. male physicians followed for more than 10 years.
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