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Laurent Viennot (Paris Diderot University, France) presented a network-based algorithm for protein module prediction based on BLAST results and applied it for a study of families of shared genetic fragments across the three domains, plasmidome and virome.
Based on BLAST results, we determined that 348 contigs are bacterial.
All amino acid sequence comparisons were based on blast results.
The remaining 23 putative homeobox genes were roughly classified based on Blast results.
It is based on BLAST results and the assignment to the NCBI taxonomy is performed using the lowest common ancestor (LCA) algorithm.
Pairs of gene-specific primers (GSP) were designed to flank each predicted insertion point based on BLAST results for TAIL-PCR amplicons.
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Since the similarity of the two clones from sample 12 was more than 85%% based on BLAST result, we propose that this sequence is a newly discovered anammox species from an agricultural environment, which is also supported by the high bootstrap values of 95 and 97 % in the phylogenetic tree.
To help visualize the presence or absence of a gene in other genomes, heatmap.2 in g-plot and R-package were also used to prepare heatmaps based on blasting results.
Based on BLAST [65] results (using default parameters with the exception –v 1 –b 1) it assigned sequences to NCBI taxonomies by employing the Lowest Common Ancestor algorithm.
a Putative identity was assigned based on BLAST analysis results.
In D. pulex, we determined the presence or absence of 25 homologs of Drosophila meiotic genes from Flybase [ 26] (Table 1) based on BLAST search results because initially we were interested in whether parthenogenetic reproduction is associated with lack of expression of meiosis-associated genes.
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